Human GREM2/Prdc ELISA Kit PicoKine®, Carrier-free

Human GREM2/Prdc ELISA Kit PicoKine® (96 Tests). Quantitate Human GREM2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

GREM2, also known as PRDC, is one of several BMP antagonists that regulate the functions of BMPs during embryonic development and tissue differentiation. BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its binding to BMP proteins. The PRDC gene is mapped to chromosome 1q43.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>142</td><td>139</td><td>149</td><td>126</td><td>139</td><td>8.33</td><td>5.9%</td></tr><tr><td>Sample 2</td><td>522</td><td>566</td><td>575</td><td>538</td><td>550</td><td>21.26</td><td>3.8%</td></tr><tr><td>Sample 3</td><td>1488</td><td>1697</td><td>1588</td><td>1757</td><td>1632</td><td>103.1</td><td>6.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Gremlin-2;Cysteine knot superfamily 1, BMP antagonist 2;DAN domain family member 3;Protein related to DAN and cerberus;GREM2;CKTSF1B2, DAND3, PRDC;

Gene Name

Gremlin-2

Gene ID

64388

UniProt

Q9H772

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R22-Q168

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<12 pg/ml

Function

Cytokine that inhibits the activity of BMP2 and BMP4 in a dose-dependent manner, and thereby modulates signaling by BMP family members. Contributes to the regulation of embryonic morphogenesis via BMP family members. Antagonizes BMP4-induced suppression of progesterone production in granulosa cells (By similarity). .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)