Mouse WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®, Carrier-free

Mouse WIF1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Wif1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>40</td><td>40</td><td>45</td><td>38</td><td>40</td><td>2.58</td><td>6.4%</td></tr><tr><td>Sample 2</td><td>298</td><td>281</td><td>269</td><td>282</td><td>282</td><td>10.3</td><td>3.6%</td></tr><tr><td>Sample 3</td><td>975</td><td>1067</td><td>1053</td><td>1054</td><td>1037</td><td>36.36</td><td>3.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Wnt inhibitory factor 1;WIF-1;Wif1;

Gene Name

Wnt inhibitory factor 1

Gene ID

24117

UniProt

Q9WUA1

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G29-W379

Tissue Specificity

Expression highest in heart and lung. Lower in brain and eye.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

23.4 pg/ml - 1500 pg/ml

Sensitivity

<10 pg/ml

Function

Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)