Mouse NOPE/IGDCC4 ELISA Kit PicoKine®, Carrier-free

Mouse NOPE/IGDCC4 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Igdcc4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

IGDCC4, also known as NOPE, plays essential roles in biologic functions of the cell surface, including cell adhesion, cell migration, and signal transduction. NOPE is identified as an oncofetal marker of murine and human hepatocellular carcinoma (HCC). Its expression is elevated in mouse and human hepatoma cell lines and in mouse hepatomas, but it is not expressed in normal liver or preneoplastic mouse HCC. Furthermore, NOPE is expressed in both AFP -positive and -negative tumors and is specifically expressed in epithelial tumor cells.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>194</td><td>209</td><td>189</td><td>183</td><td>193</td><td>9.62</td><td>4.9%</td></tr><tr><td>Sample 2</td><td>1177</td><td>1206</td><td>1298</td><td>1296</td><td>1244</td><td>53.74</td><td>4.3%</td></tr><tr><td>Sample 3</td><td>4535</td><td>4341</td><td>3824</td><td>4624</td><td>4331</td><td>310.08</td><td>7.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Immunoglobulin superfamily DCC subclass member 4;Neighbor of punc e11;Protein DDM36;Igdcc4;Ddm36, Kiaa1628, Nope;

Gene Name

Immunoglobulin superfamily DCC subclass member 4

Gene ID

56741

UniProt

Q9EQS9

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E25-H954

Tissue Specificity

Expressed in skeletal muscle, heart and brain. Brain expression is hippocampus-specific. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

125 pg/ml - 8,000 pg/ml

Sensitivity

<10 pg/ml

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)