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Rat GDF5/Bmp 14 ELISA Kit PicoKine®, Carrier-free

Rat GDF5/Bmp 14 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Gdf5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Growth/differentiation factor 5 is a protein that in humans is encoded by the GDF5 gene. GDF5 is a protein belonging to the transforming growth factor beta superfamily that is expressed in the developing central nervous system, and has a role in skeletal and joint development. It also increases the survival of neurones that respond to the neurotransmitter dopamine, and is a potential therapeutic molecule associated with Parkinson's disease.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>60</td><td>59</td><td>68</td><td>62</td><td>62</td><td>3.49</td><td>5.6%</td></tr><tr><td>Sample 2</td><td>323</td><td>317</td><td>316</td><td>281</td><td>309</td><td>16.52</td><td>5.3%</td></tr><tr><td>Sample 3</td><td>894</td><td>859</td><td>910</td><td>831</td><td>873</td><td>30.69</td><td>3.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Growth and differentiation factor-5 ;Gdf5 ;gdf-5 ;

Gene Name

growth differentiation factor 5

Gene ID

252835

UniProt

Q8K4X5

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A376-R495

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

7491 MW

Specificity

Natural and recombinant rat GDF5

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted .

Protein Name

Growth and differentiation factor-5

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