Human CD28 ELISA Kit PicoKine®, Carrier-free

Human CD28 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD28 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co- stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). The CD28 gene is mapped to 2q33-q34. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>187</td><td>181</td><td>197</td><td>178</td><td>185</td><td>7.25</td><td>3.9%</td></tr><tr><td>Sample 2</td><td>557</td><td>575</td><td>487</td><td>501</td><td>530</td><td>36.89</td><td>6.9%</td></tr><tr><td>Sample 3</td><td>1373</td><td>1278</td><td>1376</td><td>1381</td><td>1352</td><td>42.81</td><td>3.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

T-cell-specific surface glycoprotein CD28;TP44;CD28;CD28;

Gene Name

T-cell-specific surface glycoprotein CD28

Gene ID

940

UniProt

P10747

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: N19-P152

Tissue Specificity

Expressed in T-cells and plasma cells, but not in less mature B-cells.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<12 pg/ml

Function

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)