Human Fibulin-3/EFEMP1 ELISA Kit PicoKine®, Carrier-free

Human Fibulin-3/EFEMP1 ELISA Kit PicoKine® (96 Tests). Quantitate Human EFEMP1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

EGF-containing fibulin-like extracellular matrix protein 1 is a protein that in humans is encoded by the EFEMP1 gene. This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>650</td><td>662</td><td>670</td><td>638</td><td>655</td><td>12.12</td><td>1.8%</td></tr><tr><td>Sample 2</td><td>2080</td><td>2103</td><td>2020</td><td>2397</td><td>2150</td><td>145.78</td><td>6.7%</td></tr><tr><td>Sample 3</td><td>10283</td><td>8683</td><td>9084</td><td>9862</td><td>9478</td><td>629.03</td><td>6.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

EGF-containing fibulin-like extracellular matrix protein 1;Extracellular protein S1-5;Fibrillin-like protein;Fibulin-3;FIBL-3;EFEMP1;FBLN3, FBNL;

Gene Name

EGF-containing fibulin-like extracellular matrix protein 1

Gene ID

2202

UniProt

Q12805

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q18-F493

Tissue Specificity

In the eye, associated with photoreceptor outer and inner segment regions, the nerve fiber layer, outer nuclear layer and inner and outer plexiform layers of the retina. .

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Function

Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

54641 MW

Specificity

Natural and recombinant human Fibulin-3

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted, extracellular space . Secreted, extracellular space, extracellular matrix . Localizes to the lamina propria underneath the olfactory epithelium. .