Human Hemopexin ELISA Kit PicoKine®, Carrier-free

Human Hemopexin ELISA Kit PicoKine® (96 Tests). Quantitate Human HPX in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Hemopexin (or haemopexin; HPX), also known as beta-1B-glycoprotein, is a protein that in humans is encoded by the HPX gene and belongs to hemopexin family of proteins. Hemopexin binds heme with the highest affinity of any known protein. Its function is scavenging the heme released or lost by the turnover of heme proteins such as hemoglobin and thus protects the body from the oxidative damage that free heme can cause. In addition, hemopexin releases its bound ligand for internalisation upon interacting with a specific receptor situated on the surface of liver cells. This function of hemopexin is to preserve the body's iron.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>7144</td><td>6667</td><td>7002</td><td>7690</td><td>7125</td><td>368.94</td><td>5.1%</td></tr><tr><td>Sample 2</td><td>26562</td><td>26917</td><td>29717</td><td>26482</td><td>27419</td><td>1336.52</td><td>4.8%</td></tr><tr><td>Sample 3</td><td>114865</td><td>125689</td><td>107714</td><td>123178</td><td>117861</td><td>7097.28</td><td>6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Hemopexin;Beta-1B-glycoprotein;HPX;

Gene Name

Hemopexin

Gene ID

3263

UniProt

P02790

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T24-H462

Tissue Specificity

Expressed by the liver and secreted in plasma.

Applications

ELISA

Standard

200 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

3.12 ng/ml - 200 ng/ml

Sensitivity

<50 pg/ml

Function

Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)