Human HE4 ELISA Kit PicoKine®, Carrier-free

Human HE4 ELISA Kit PicoKine® (96 Tests). Quantitate Human WFDC2 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

WAP four-disulfide core domain protein 2, also known as Human Epididymis Protein 4 (HE4), is a protein that in humans is encoded by the WFDC2 gene. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>246</td><td>276</td><td>254</td><td>281</td><td>264</td><td>14.63</td><td>5.5%</td></tr><tr><td>Sample 2</td><td>1139</td><td>1088</td><td>1273</td><td>1192</td><td>1173</td><td>68.45</td><td>5.8%</td></tr><tr><td>Sample 3</td><td>4402</td><td>3964</td><td>4189</td><td>4282</td><td>4209</td><td>160.47</td><td>3.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

WAP four-disulfide core domain protein 2;Epididymal secretory protein E4;Major epididymis-specific protein E4;Putative protease inhibitor WAP5;WFDC2;HE4, WAP5;

Gene Name

WAP four-disulfide core domain protein 2

Gene ID

10406

UniProt

Q14508

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E31-F124

Tissue Specificity

Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<15 pg/ml

Function

Broad range protease inhibitor. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)