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Human Marapsin/Pancresin ELISA Kit PicoKine®, Carrier-free

Human Marapsin/Pancresin ELISA Kit PicoKine® (96 Tests). Quantitate Human PRSS27 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Pancreasin, also known as PRSS27 or Marapsin, is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. It belongs to the group-1 subfamily of serine proteases. The pancreasin gene is mapped to chromosome 16p13.3. Pancreasin is resistant to inactivation by large molecular mass trypsin inhibitors, but it is sensitive to benzamidine and leupeptin.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>167</td><td>176</td><td>171</td><td>165</td><td>169</td><td>4.2</td><td>2.4%</td></tr><tr><td>Sample 2</td><td>1395</td><td>1278</td><td>1352</td><td>1276</td><td>1325</td><td>50.59</td><td>3.8%</td></tr><tr><td>Sample 3</td><td>5662</td><td>5287</td><td>5308</td><td>6068</td><td>5581</td><td>318.07</td><td>5.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Serine protease 27;3.4.21.-;Marapsin;Pancreasin;PRSS27;MPN;UNQ1884/PRO4327;

Gene Name

Serine protease 27

Gene ID

83886

UniProt

Q9BQR3

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M35-K290

Tissue Specificity

Expressed predominantly in the pancreas. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants and serum.

Detection Range

125 pg/ml - 8,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

31940 MW

Specificity

Natural and recombinant human Marapsin

Subcellular Location

Secreted.

Protein Name

Serine protease 27

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