Rat MMP-9 ELISA Kit PicoKine®, Carrier-free

Rat <a href="https://www.bosterbio.com/human-mmp-9-picokine-trade-elisa-kit-ek0465-boster.html" style="color:#EA8D28">MMP-9 ELISA Kit</a> PicoKine® (96 Tests). Quantitate Rat Mmp9 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Matrix metallopeptidase 9 (MMP-9) is also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>429</td><td>457</td><td>420</td><td>449</td><td>438</td><td>14.87</td><td>3.3%</td></tr><tr><td>Sample 2</td><td>2362</td><td>2453</td><td>2332</td><td>2307</td><td>2363</td><td>55.22</td><td>2.3%</td></tr><tr><td>Sample 3</td><td>8880</td><td>10067</td><td>9013</td><td>9434</td><td>9348</td><td>462.49</td><td>4.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Matrix metalloproteinase-9;MMP-9;3.4.24.35;92 kDa gelatinase;92 kDa type IV collagenase;Gelatinase B;GELB;Mmp9;

Gene Name

Matrix metalloproteinase-9

Gene ID

81687

UniProt

P50282

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L35-P708

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin).

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<15 pg/ml

Function

Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (By similarity). .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)