Human CA125/MUC16 ELISA Kit PicoKine®, Carrier-free

CA-125, also known as mucin 16 or MUC16, is a protein that in humans is encoded by the MUC16 gene. MUC16 is a member of the mucin family glycoproteins. It is a component of the ocular surface, the respiratory tract and the female reproductive tract epithelia. Since MUC16 is highly glycosylated it creates a hydrophilic environment that acts as a lubricating barrier against foreign particles and infectious agents on the apical membrane of epithelial cells. Also, the cytoplasmic tail of MUC16 has been shown to interact with cytoskeleton by binding members of the ERM protein family. The expression of mucin 16 has been shown to be altered in dry eye, cystic fibrosis, and several types of cancers.

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (U/ml)</th><th>Lot 2 (U/ml)</th><th>Lot 3 (U/ml)</th><th>Lot 4 (U/ml)</th><th>Mean (U/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>6</td><td>7</td><td>7</td><td>7</td><td>7</td><td>0.41</td><td>6.8%</td></tr><tr><td>Sample 2</td><td>8</td><td>8</td><td>11</td><td>11</td><td>10</td><td>0.31</td><td>3.3%</td></tr><tr><td>Sample 3</td><td>71</td><td>64</td><td>98</td><td>79</td><td>78</td><td>2.56</td><td>3.3%</td></tr></table>*number of samples for each test n=16.

Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane; Single-pass type I membrane protein. Secreted, extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.