Human SFRP5/Sarp3 ELISA Kit PicoKine®, Carrier-free

Human SFRP5/Sarp3 ELISA Kit PicoKine® (96 Tests). Quantitate Human SFRP5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>329</td><td>353</td><td>327</td><td>347</td><td>339</td><td>11.22</td><td>3.3%</td></tr><tr><td>Sample 2</td><td>1068</td><td>1216</td><td>1033</td><td>1211</td><td>1132</td><td>82.45</td><td>7.2%</td></tr><tr><td>Sample 3</td><td>4969</td><td>5003</td><td>5645</td><td>5635</td><td>5313</td><td>327.23</td><td>6.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Secreted frizzled-related protein 5;sFRP-5;Frizzled-related protein 1b;FRP-1b;Secreted apoptosis-related protein 3;SARP-3;SFRP5;FRP1B, SARP3;

Gene Name

Secreted frizzled-related protein 5

Gene ID

6425

UniProt

Q5T4F7

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: E30-H317

Tissue Specificity

Highly expressed in the retinal pigment epithelium (RPE) and pancreas. Weak expression in heart, liver and muscle. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<15 pg/ml

Function

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)