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Human SAA/SAA1 ELISA Kit PicoKine®, Carrier-free

Human SAA/SAA1 ELISA Kit PicoKine® (96 Tests). Quantitate Human SAA1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 150pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Serum amyloid A protein (SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>3858</td><td>3499</td><td>3839</td><td>4125</td><td>3830</td><td>222.17</td><td>5.8%</td></tr><tr><td>Sample 2</td><td>13218</td><td>13736</td><td>13026</td><td>14070</td><td>13512</td><td>413.57</td><td>3%</td></tr><tr><td>Sample 3</td><td>48138</td><td>46567</td><td>52708</td><td>53611</td><td>50256</td><td>2973.33</td><td>5.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Serum amyloid A-1 protein;SAA;Amyloid protein A;Amyloid fibril protein AA;Serum amyloid protein A (2-104);Serum amyloid protein A (3-104);Serum amyloid protein A (2-103);Serum amyloid protein A (2-102);Serum amyloid protein A (4-101);SAA1;

Gene Name

Serum amyloid A-1 protein

Gene ID

6288

UniProt

P0DJI8

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S20-Y122

Tissue Specificity

Expressed by the liver; secreted in plasma (at protein level). .

Applications

ELISA

Standard

100 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

1.56 ng/ml - 100 ng/ml

Sensitivity

<150 pg/ml

Function

Major acute phase protein.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

13532 MW

Specificity

Natural and recombinant human SAA

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted .

Protein Name

Serum amyloid A-1 protein

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