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Human Oncomodulin ELISA Kit PicoKine®, Carrier-free

Human Oncomodulin ELISA Kit PicoKine® (96 Tests). Quantitate Human OCM in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Oncomodulin, also known as OCM, is an oncodevelopmental protein found in early embryonic cells in the placenta and also in tumors. The human gene for oncomodulin is located on chromosome 7. It is a parvalbumin- family calcium-binding protein expressed and secreted by macrophages that typically traffic to tissue as an inflammatory response or after injury. Oncomodulin promotes axon regeneration in retinal ganglion cells and maintains functioning in mouse cochlear hair cells.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>102</td><td>101</td><td>94</td><td>100</td><td>99</td><td>3.11</td><td>3.1%</td></tr><tr><td>Sample 2</td><td>272</td><td>264</td><td>287</td><td>282</td><td>276</td><td>8.89</td><td>3.2%</td></tr><tr><td>Sample 3</td><td>897</td><td>877</td><td>847</td><td>824</td><td>861</td><td>27.91</td><td>3.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

Oncomodulin-1;OM;Parvalbumin beta;OCM;OCM1, OCMN;

Gene Name

Oncomodulin-1

Gene ID

654231

UniProt

P0CE72

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S2-S109

Applications

ELISA

Standard

2 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Has some calmodulin-like activity with respect to enzyme activation and growth regulation. Binds two calcium ions.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

12184 MW

Specificity

Natural and recombinant human Oncomodulin

Anticoagulant

heparin or EDTA

Protein Name

Oncomodulin-1

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