Mouse SPINT1/HAI-1 ELISA Kit PicoKine®, Carrier-free

Mouse SPINT1/HAI-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Spint1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Kunitz-type protease inhibitor 1 is an enzyme that in humans is encoded by the SPINT1 gene. It is a member of the Kunitz family of serine protease inhibitors. The SPINT1 gene encodes an inhibitor of hepatocyte growth factor activator (HGFAC) and matriptase and is involved in degradation of the extracellular matrix. SPINT1 is produced as an active membrane-associated protein and is proteolytically cleaved and secreted. Alternative splicing results in multiple variants encoding different isoforms.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>199</td><td>180</td><td>196</td><td>188</td><td>190</td><td>7.39</td><td>3.8%</td></tr><tr><td>Sample 2</td><td>707</td><td>699</td><td>666</td><td>710</td><td>695</td><td>17.5</td><td>2.5%</td></tr><tr><td>Sample 3</td><td>1860</td><td>1696</td><td>2012</td><td>1755</td><td>1830</td><td>120</td><td>6.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Kunitz-type protease inhibitor 1;Hepatocyte growth factor activator inhibitor type 1;HAI-1;Spint1;Hai1;

Gene Name

Kunitz-type protease inhibitor 1

Gene ID

20732

UniProt

Q9R097

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E30-L507

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<12 pg/ml

Function

Inhibitor of HGF activator. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)