Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine®, Carrier-free

Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine® (96 Tests). Quantitate Human Tryptase/TPSAB1,B2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>276</td><td>255</td><td>284</td><td>276</td><td>272</td><td>10.75</td><td>3.9%</td></tr><tr><td>Sample 2</td><td>1887</td><td>1991</td><td>1959</td><td>1839</td><td>1919</td><td>59.59</td><td>3.1%</td></tr><tr><td>Sample 3</td><td>5608</td><td>5276</td><td>5559</td><td>5824</td><td>5566</td><td>195.24</td><td>3.5%</td></tr></table>*number of samples for each test n=16.

Gene Name

Tryptase/TPSAB1,B2

Gene ID

7177

UniProt

Q15661

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Met1-Pro275

Tissue Specificity

TPSAB1: Isoform 1 and isoform 2 are expressed in lung,stomach, spleen, heart and skin; in these tissues, isoform 1 ispredominant. Isoform 2 is expressed in aorta, spleen, and breasttumor, with highest levels in the endothelial cells of some bloodvessels surrounding the aorta, as well as those surrounding thetumor and low levels, if any, in mast cells (at protein level).

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<15 pg/ml

Function

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)