Mouse IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine®, Carrier-free

Mouse IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ids in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>292</td><td>332</td><td>309</td><td>275</td><td>302</td><td>21.08</td><td>6.9%</td></tr><tr><td>Sample 2</td><td>1534</td><td>1618</td><td>1672</td><td>1683</td><td>1626</td><td>58.92</td><td>3.6%</td></tr><tr><td>Sample 3</td><td>5259</td><td>5568</td><td>5740</td><td>5102</td><td>5417</td><td>250.65</td><td>4.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Iduronate 2-sulfatase;3.1.6.13;Alpha-L-iduronate sulfate sulfatase;Ids;

Gene Name

Iduronate 2-sulfatase

Gene ID

15931

UniProt

Q08890

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T36-P552

Tissue Specificity

Found to be expressed in alpha and beta pancreatic cells. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<15 pg/ml

Function

Required for the lysosomal degradation of heparan sulfate and dermatan sulfate. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)