Mouse CD23/FCER2 ELISA Kit PicoKine®, Carrier-free

Mouse CD23/FCER2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Fcer2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the CD23 gene was assigned to 19p13.3. And CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>256</td><td>296</td><td>293</td><td>254</td><td>274</td><td>19.79</td><td>7.2%</td></tr><tr><td>Sample 2</td><td>1831</td><td>1672</td><td>1609</td><td>1822</td><td>1733</td><td>95.68</td><td>5.5%</td></tr><tr><td>Sample 3</td><td>5853</td><td>5387</td><td>5221</td><td>5268</td><td>5432</td><td>250.34</td><td>4.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Low affinity immunoglobulin epsilon Fc receptor;Fc-epsilon-RII;Lymphocyte IgE receptor;CD23;Fcer2;Fcer2a;

Gene Name

Low affinity immunoglobulin epsilon Fc receptor

Gene ID

14128

UniProt

P20693

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E50-P331

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<15 pg/ml

Function

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)