Human LBP ELISA Kit PicoKine®, Carrier-free

Human LBP ELISA Kit PicoKine® (96 Tests). Quantitate Human LBP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1485</td><td>1561</td><td>1630</td><td>1640</td><td>1579</td><td>62.21</td><td>3.9%</td></tr><tr><td>Sample 2</td><td>4533</td><td>5189</td><td>4438</td><td>4703</td><td>4715</td><td>289.25</td><td>6.1%</td></tr><tr><td>Sample 3</td><td>23562</td><td>22985</td><td>21907</td><td>22035</td><td>22622</td><td>683.95</td><td>3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Lipopolysaccharide-binding protein;LBP;LBP;

Gene Name

Lipopolysaccharide-binding protein

Gene ID

3929

UniProt

P18428

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A26-V481

Tissue Specificity

Detected in blood serum (at protein level). .

Applications

ELISA

Standard

50 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

0.78 ng/ml - 50 ng/ml

Sensitivity

<50 pg/ml

Function

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria (PubMed:7517398, PubMed:24120359). Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide (PubMed:7517398, PubMed:24120359). .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)