Human CD30/TNFRSF8 ELISA Kit PicoKine®, Carrier-free

Human CD30/TNFRSF8 ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>321</td><td>324</td><td>313</td><td>319</td><td>319</td><td>4.02</td><td>1.2%</td></tr><tr><td>Sample 2</td><td>1322</td><td>1420</td><td>1463</td><td>1452</td><td>1414</td><td>55.55</td><td>3.9%</td></tr><tr><td>Sample 3</td><td>3947</td><td>3780</td><td>4218</td><td>4450</td><td>4098</td><td>256.04</td><td>6.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

Tumor necrosis factor receptor superfamily member 8;CD30L receptor;Ki-1 antigen;Lymphocyte activation antigen CD30;CD30;TNFRSF8;CD30, D1S166E;

Gene Name

Tumor necrosis factor receptor superfamily member 8

Gene ID

943

UniProt

P28908

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F19-K379

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)