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Human ADAMTS13 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK0927-carrier-free

Size: 96 Well With Removable Strips

Human ADAMTS13 ELISA Kit PicoKine® (96 Tests). Quantitate Human ADAMTS13 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP). ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1395</td><td>1323</td><td>1392</td><td>1567</td><td>1419</td><td>90.03</td><td>6.3%</td></tr><tr><td>Sample 2</td><td>7236</td><td>8286</td><td>7812</td><td>8064</td><td>7849</td><td>391.89</td><td>4.9%</td></tr><tr><td>Sample 3</td><td>20816</td><td>20291</td><td>22433</td><td>22964</td><td>21626</td><td>1104.51</td><td>5.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

A disintegrin and metalloproteinase with thrombospondin motifs 13;ADAM-TS 13;ADAM-TS13;ADAMTS-13;3.4.24.87;von Willebrand factor-cleaving protease;vWF-CP;vWF-cleaving protease;ADAMTS13;C9orf8;UNQ6102/PRO20085;

Gene Name

A disintegrin and metalloproteinase with thrombospondin motifs 13

Gene ID

11093

UniProt

Q76LX8

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q34-W688

Tissue Specificity

Plasma. Expressed primarily in liver. .

Applications

ELISA

Standard

50 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

0.78 ng/ml - 50 ng/ml

Sensitivity

<20 pg/ml

Function

Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

153604 MW

Specificity

Natural and recombinant human ADAMTS13

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Secreted . Secretion enhanced by O-fucosylation of TSP type-1 repeats.

Protein Name

A disintegrin and metalloproteinase with thrombospondin motifs 13

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