Human PEDF/SerpinF1 ELISA Kit PicoKine®, Carrier-free

Human PEDF/SerpinF1 ELISA Kit PicoKine® (96 Tests). Quantitate Human SERPINF1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Pigment epithelium-derived factor (PEDF) is also known as serpin F1 (SERPINF1). In humans, it is encoded by the SERPINF1 gene. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. It suppresses retinal neovascularization and endothelial cell proliferation. And Antiangiogenic function is also conferred by PEDF through inhibition of both VEGFR-1 and VEGFR-2. In addition, the antitumorigenic effects of PEDF are not only due to inhibition of supporting vasculature, but also due to effects on the cancer cells themselves. PEDF is shown to inhibit cancer cell proliferation and increase apoptosis via the FAS/FASL pathway. Expression of PEDF in the human retina is found at 7.4 weeks of gestation, suggesting it may play a role in retinal neuron differentiation.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>286</td><td>285</td><td>271</td><td>293</td><td>284</td><td>13.05</td><td>4.6%</td></tr><tr><td>Sample 2</td><td>780</td><td>796</td><td>821</td><td>805</td><td>801</td><td>33.62</td><td>4.2%</td></tr><tr><td>Sample 3</td><td>2612</td><td>2744</td><td>2752</td><td>2653</td><td>2690</td><td>104.92</td><td>3.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Pigment epithelium-derived factor;PEDF;Cell proliferation-inducing gene 35 protein;EPC-1;Serpin F1;SERPINF1;PEDF;PIG35;

Gene Name

Pigment epithelium-derived factor

Gene ID

5176

UniProt

P36955

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-P418

Tissue Specificity

Retinal pigment epithelial cells and blood plasma. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<20 pg/ml

Function

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)