Human DCR3/TNFRSF6B ELISA Kit PicoKine®, Carrier-free

Human DCR3/TNFRSF6B ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF6B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Decoy receptor 3 (Dcr3), also known as tumor necrosis factor receptor superfamily member 6B (TNFRSF6B), TR6 and M68, is a soluble protein of the tumor necrosis factor receptor superfamily which inhibits Fas ligand-inducedapoptosis. This gene belongs to the tumor necrosis factor receptor superfamily. It acts as a decoy receptor that competes with death receptors for ligand binding. The encoded protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death and T cell activation as well as to induce angiogenesis via neutralization of TL1A. Overexpression of this gene has been noted in various tumors e.g. gastrointestinal tract tumors, and it is located in a gene-rich cluster on chromosome 20, with other potentially tumor-related genes.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>272</td><td>246</td><td>272</td><td>280</td><td>267</td><td>12.83</td><td>4.8%</td></tr><tr><td>Sample 2</td><td>1644</td><td>1634</td><td>1754</td><td>1881</td><td>1728</td><td>99.97</td><td>5.7%</td></tr><tr><td>Sample 3</td><td>5011</td><td>5645</td><td>5121</td><td>5650</td><td>5356</td><td>293.34</td><td>5.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Tumor necrosis factor receptor superfamily member 6B;Decoy receptor 3;DcR3;Decoy receptor for Fas ligand;M68;TNFRSF6B;DCR3, TR6;UNQ186/PRO212;

Gene Name

Tumor necrosis factor receptor superfamily member 6B

Gene ID

8771

UniProt

O95407

Reactivity

Human

Cross Reactivity

No cross reactivity with other proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V24-H300

Tissue Specificity

Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)