Mouse Cripto/TDGF1 ELISA Kit PicoKine®, Carrier-free

Mouse Cripto/TDGF1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tdgf1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Teratocarcinoma-derived growth factor 1 is a protein that in humans is encoded by the TDGF1 gene. It is mapped to 3p23-p21. The protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic developmentand tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>110</td><td>110</td><td>114</td><td>115</td><td>112</td><td>2.27</td><td>2%</td></tr><tr><td>Sample 2</td><td>661</td><td>633</td><td>642</td><td>685</td><td>655</td><td>19.92</td><td>3%</td></tr><tr><td>Sample 3</td><td>1213</td><td>1047</td><td>1166</td><td>1231</td><td>1164</td><td>71.73</td><td>6.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Teratocarcinoma-derived growth factor;Cripto growth factor;Epidermal growth factor-like Cripto protein;Tdgf1;Cripto;

Gene Name

Teratocarcinoma-derived growth factor

Gene ID

21667

UniProt

P51865

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R26-Q150

Tissue Specificity

Expressed at low level in specific organs of the adult animal such as spleen, heart, lung and brain. During gastrulation, expressed in the forming mesoderm. In later stages of the developing heart, expression is restricted to the truncus arteriosus.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)