Human Tenascin-C/TNC ELISA Kit PicoKine®, Carrier-free

Human Tenascin-C/TNC ELISA Kit PicoKine® (96 Tests). Quantitate Human TNC in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Tenascin C (TN-C) is a glycoprotein that in humans is encoded by the TNC gene. It is expressed in the extracellular matrix of various tissues during development, disease or injury, and in restricted neurogenic areas of the central nervous system. Tenascin-C is the founding member of the gene family (Tenascin). In the embryo it is made by migrating cells like the neural crest; it is also abundant in developing tendons, bone and cartilage. TN-C clearly plays a role in cell signaling as evidenced by its ability to be induced during events such as trauma, inflammation, or cancer development. Also, TN-C is important in regulating cell proliferation and migration, especially during developmental differentiation and wound healing.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>94</td><td>114</td><td>94</td><td>112</td><td>103</td><td>9.52</td><td>9.2%</td></tr><tr><td>Sample 2</td><td>868</td><td>820</td><td>876</td><td>826</td><td>847</td><td>24.75</td><td>2.9%</td></tr><tr><td>Sample 3</td><td>3019</td><td>2632</td><td>2824</td><td>3047</td><td>2880</td><td>167.21</td><td>5.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Tenascin;TN;Cytotactin;GMEM;GP 150-225;Glioma-associated-extracellular matrix antigen;Hexabrachion;JI;Myotendinous antigen;Neuronectin;Tenascin-C;TN-C;TNC;HXB;

Gene Name

Tenascin

Gene ID

3371

UniProt

P24821

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G23-P625

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

93.7 pg/ml - 6,000 pg/ml

Sensitivity

<10 pg/ml

Function

Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)