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Human DAN/NBL1 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK0746-carrier-free

Size: 96 Well With Removable Strips

Human DAN/NBL1 ELISA Kit PicoKine® (96 Tests). Quantitate Human NBL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>158</td><td>176</td><td>186</td><td>157</td><td>169</td><td>12.27</td><td>7.2%</td></tr><tr><td>Sample 2</td><td>931</td><td>1035</td><td>907</td><td>1065</td><td>984</td><td>66.89</td><td>6.7%</td></tr><tr><td>Sample 3</td><td>5529</td><td>5905</td><td>6039</td><td>5194</td><td>5666</td><td>330.83</td><td>5.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Neuroblastoma suppressor of tumorigenicity 1;DAN domain family member 1;Protein N03;Zinc finger protein DAN;NBL1;DAN, DAND1;

Gene Name

Neuroblastoma suppressor of tumorigenicity 1

Gene ID

4681

UniProt

P41271

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A17-D181

Tissue Specificity

Most abundant in normal lung and meningioma.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

125 pg/ml - 8,000 pg/ml

Sensitivity

<10 pg/ml

Function

Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

19408 MW

Specificity

Natural and recombinant human DAN

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Protein Name

Neuroblastoma suppressor of tumorigenicity 1

Price

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