Human DAN/NBL1 ELISA Kit PicoKine®, Carrier-free

Human DAN/NBL1 ELISA Kit PicoKine® (96 Tests). Quantitate Human NBL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>158</td><td>176</td><td>186</td><td>157</td><td>169</td><td>12.27</td><td>7.2%</td></tr><tr><td>Sample 2</td><td>931</td><td>1035</td><td>907</td><td>1065</td><td>984</td><td>66.89</td><td>6.7%</td></tr><tr><td>Sample 3</td><td>5529</td><td>5905</td><td>6039</td><td>5194</td><td>5666</td><td>330.83</td><td>5.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Neuroblastoma suppressor of tumorigenicity 1;DAN domain family member 1;Protein N03;Zinc finger protein DAN;NBL1;DAN, DAND1;

Gene Name

Neuroblastoma suppressor of tumorigenicity 1

Gene ID

4681

UniProt

P41271

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A17-D181

Tissue Specificity

Most abundant in normal lung and meningioma.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

125 pg/ml - 8,000 pg/ml

Sensitivity

<10 pg/ml

Function

Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)