Mouse CD86/B7-2 ELISA Kit PicoKine®, Carrier-free

Mouse CD86/B7-2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd86 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>179</td><td>175</td><td>168</td><td>168</td><td>172</td><td>4.71</td><td>2.7%</td></tr><tr><td>Sample 2</td><td>772</td><td>787</td><td>740</td><td>767</td><td>766</td><td>16.97</td><td>2.2%</td></tr><tr><td>Sample 3</td><td>1639</td><td>1511</td><td>1669</td><td>1837</td><td>1664</td><td>116.17</td><td>6.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

T-lymphocyte activation antigen CD86;Activation B7-2 antigen;Early T-cell costimulatory molecule 1;ETC-1;CD86;Cd86;

Gene Name

T-lymphocyte activation antigen CD86

Gene ID

12524

UniProt

P42082

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V26-G245

Tissue Specificity

Expressed on activated B-cells.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants and serum.

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)