Human IGFBP4 PicoKine® ELISA Kit, Carrier-free

Human IGFBP4 PicoKine® ELISA Kit (96 Tests). Quantitate Human IGFBP4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. In addition, IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. It is secreted by all colon cancer cells.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>634</td><td>669</td><td>599</td><td>583</td><td>621</td><td>33.16</td><td>5.3%</td></tr><tr><td>Sample 2</td><td>3283</td><td>3066</td><td>3571</td><td>3160</td><td>3270</td><td>190.05</td><td>5.8%</td></tr><tr><td>Sample 3</td><td>15689</td><td>15202</td><td>16042</td><td>14388</td><td>15330</td><td>620.39</td><td>4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Insulin-like growth factor-binding protein 4;IBP-4;IGF-binding protein 4;IGFBP-4;IGFBP4;IBP4;

Gene Name

Insulin-like growth factor-binding protein 4

Gene ID

3487

UniProt

P22692

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D22-E258

Applications

ELISA

Standard

32 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

500 pg/ml - 32,000 pg/ml

Sensitivity

<20 pg/ml

Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)