Mouse MBL2/MPB-C ELISA Kit PicoKine®, Carrier-free

Mouse MBL2/MPB-C ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Mbl2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>656</td><td>711</td><td>654</td><td>663</td><td>671</td><td>23.33</td><td>3.4%</td></tr><tr><td>Sample 2</td><td>1840</td><td>1861</td><td>1847</td><td>1853</td><td>1850</td><td>7.72</td><td>0.4%</td></tr><tr><td>Sample 3</td><td>9232</td><td>10414</td><td>9072</td><td>9277</td><td>9498</td><td>533.88</td><td>5.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Mannose-binding protein C;MBP-C;Mannan-binding protein;RA-reactive factor P28A subunit;RARF/P28A;Mbl2;

Gene Name

Mannose-binding protein C

Gene ID

17195

UniProt

P41317

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-D244

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Function

Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages (By similarity). .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)