Mouse Tissue Factor/F3 ELISA Kit PicoKine®, Carrier-free

Mouse Tissue Factor/F3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse F3 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. Tissue factor gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>31</td><td>36</td><td>32</td><td>30</td><td>32</td><td>2.27</td><td>7%</td></tr><tr><td>Sample 2</td><td>167</td><td>171</td><td>178</td><td>166</td><td>170</td><td>4.71</td><td>2.7%</td></tr><tr><td>Sample 3</td><td>444</td><td>389</td><td>387</td><td>433</td><td>413</td><td>25.55</td><td>6.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Tissue factor;TF;Coagulation factor III;CD142;F3;Cf-3, Cf3;

Gene Name

Tissue factor

Gene ID

14066

UniProt

P20352

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A29-E251

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and cell lysates.

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Function

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)