Human Syndecan-4/SDC4 ELISA Kit PicoKine®, Carrier-free

Human Syndecan-4/SDC4 ELISA Kit PicoKine® (96 Tests). Quantitate Human SDC4 in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC). The variable domain of syndecan-4 could be a site of self-association. What’s more, Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>94</td><td>82</td><td>81</td><td>78</td><td>83</td><td>6.09</td><td>7.3%</td></tr><tr><td>Sample 2</td><td>673</td><td>735</td><td>621</td><td>630</td><td>664</td><td>45.06</td><td>6.7%</td></tr><tr><td>Sample 3</td><td>2108</td><td>2092</td><td>1869</td><td>1903</td><td>1993</td><td>107.82</td><td>5.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Syndecan-4;SYND4;Amphiglycan;Ryudocan core protein;SDC4;

Gene Name

Syndecan-4

Gene ID

6385

UniProt

P31431

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-E145

Tissue Specificity

Expressed in epithelial and fibroblastic cells. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates and serum.

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Cell surface proteoglycan that bears heparan sulfate.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)