Human SP-D / Surfactant protein D ELISA Kit PicoKine®, Carrier-free

Human SP-D / Surfactant protein D ELISA Kit PicoKine® (96 Tests). Quantitate Human SFTPD in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What’s more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1547</td><td>1291</td><td>1390</td><td>1463</td><td>1422</td><td>94.19</td><td>6.6%</td></tr><tr><td>Sample 2</td><td>3580</td><td>3482</td><td>3689</td><td>3752</td><td>3625</td><td>103.31</td><td>2.8%</td></tr><tr><td>Sample 3</td><td>25269</td><td>29048</td><td>29153</td><td>26765</td><td>27558</td><td>1630.37</td><td>5.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Pulmonary surfactant-associated protein D;PSP-D;SP-D;Collectin-7;Lung surfactant protein D;SFTPD;COLEC7, PSPD, SFTP4;

Gene Name

Pulmonary surfactant-associated protein D

Gene ID

6441

UniProt

P35247

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A21-F375

Tissue Specificity

Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes). .

Applications

ELISA

Standard

40 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

625 pg/ml - 40,000 pg/ml

Sensitivity

<20 pg/ml

Function

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)