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Human SDC1 / Syndecan-1 / CD138 ELISA Kit PicoKine®, Carrier-free

Human SDC1 / Syndecan-1 / CD138 ELISA Kit PicoKine® (96 Tests). Quantitate Human SDC1 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What’s more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>632</td><td>571</td><td>608</td><td>604</td><td>603</td><td>21.72</td><td>3.6%</td></tr><tr><td>Sample 2</td><td>2595</td><td>2506</td><td>2397</td><td>2686</td><td>2546</td><td>107</td><td>4.2%</td></tr><tr><td>Sample 3</td><td>8396</td><td>8079</td><td>9304</td><td>8737</td><td>8629</td><td>453.89</td><td>5.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

Syndecan-1;SYND1;CD138;SDC1;SDC;

Gene Name

Syndecan-1

Gene ID

6382

UniProt

P18827

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q23-G254

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants and serum.

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Function

Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

32462 MW

Specificity

Natural and recombinant human Syndecan-1

Subcellular Location

Membrane; Single-pass type I membrane protein. Secreted. Shedding of the ectodomain produces a soluble form.

Sequence Similarities

Belongs to the syndecan proteoglycan family.

Protein Name

Syndecan-1

Frequently Asked Questions

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