Human Mesothelin ELISA Kit PicoKine®, Carrier-free

Human Mesothelin ELISA Kit PicoKine® (96 Tests). Quantitate Human MSLN in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Mesothelin, also known as MSLN or MPF is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>307</td><td>282</td><td>307</td><td>344</td><td>310</td><td>22.12</td><td>7.1%</td></tr><tr><td>Sample 2</td><td>1077</td><td>1082</td><td>1223</td><td>1218</td><td>1150</td><td>70.54</td><td>6.1%</td></tr><tr><td>Sample 3</td><td>6075</td><td>5852</td><td>5500</td><td>5776</td><td>5800</td><td>205.48</td><td>3.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Mesothelin;CAK1 antigen;Pre-pro-megakaryocyte-potentiating factor;Megakaryocyte-potentiating factor;MPF;Mesothelin, cleaved form;MSLN;MPF;

Gene Name

Mesothelin

Gene ID

10232

UniProt

Q13421

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E296-G580

Tissue Specificity

Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Membrane-anchored forms may play a role in cellular adhesion.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)