Mouse TIMP-4 ELISA Kit PicoKine®, Carrier-free

Mouse TIMP-4 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Timp4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. TIMP4 gene is mapped to chromosome 3p25. TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. The gene is expressed as a 1.4-kb transcript abundant in heart and present at low levels in several other tissues. Expressed TIMP4 inhibits MMPs in vitro. What’s more, TIMP-4 binds both progelatinase A and the C domain in a similar manner to that of TIMP-2.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>131</td><td>113</td><td>132</td><td>117</td><td>123</td><td>8.37</td><td>6.8%</td></tr><tr><td>Sample 2</td><td>690</td><td>674</td><td>647</td><td>709</td><td>680</td><td>22.72</td><td>3.3%</td></tr><tr><td>Sample 3</td><td>2000</td><td>2212</td><td>1975</td><td>2086</td><td>2068</td><td>92.64</td><td>4.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Metalloproteinase inhibitor 4;Tissue inhibitor of metalloproteinases 4;TIMP-4;Timp4;

Gene Name

Metalloproteinase inhibitor 4

Gene ID

110595

UniProt

Q9JHB3

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: C30-P224

Tissue Specificity

Expressed in brain, heart, ovary and skeletal muscle.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

78 pg/ml - 5,000 pg/ml

Sensitivity

<10 pg/ml

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)