Mouse GPNMB/Osteoactivin ELISA Kit PicoKine®, Carrier-free

Mouse GPNMB/Osteoactivin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Gpnmb in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Transmembrane glycoprotein NMB is a protein that is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis (soft callus) and osteogenesis (hard callus) compared to the intact femurs that is why Osteoactivin (OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>265</td><td>290</td><td>285</td><td>290</td><td>282</td><td>10.3</td><td>3.6%</td></tr><tr><td>Sample 2</td><td>1462</td><td>1602</td><td>1474</td><td>1607</td><td>1536</td><td>68.4</td><td>4.4%</td></tr><tr><td>Sample 3</td><td>4620</td><td>4438</td><td>4374</td><td>4575</td><td>4501</td><td>99.66</td><td>2.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

Transmembrane glycoprotein NMB;DC-HIL;Dendritic cell-associated transmembrane protein;Osteoactivin;Gpnmb;Dchil, Hgfin, Nmb;

Gene Name

Transmembrane glycoprotein NMB

Gene ID

93695

UniProt

Q99P91

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: K23-N502

Tissue Specificity

In early development, mRNA is detected at high levels in the outer layer of the retina. Later in development expression gets restricted to the retinal pigment epithelium and iris. Overexpressed in mammary cancer cells possessing a strong bone metastatic phenotype. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Could be a melanogenic enzyme. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

63676 MW

Specificity

Natural and recombinant mouse Osteoactivin

Anticoagulant

heparin or EDTA

Subcellular Location

Cell membrane ; Single-pass type I membrane protein . Endomembrane system ; Single-pass type I membrane protein . Cytoplasmic vesicle membrane ; Single-pass type I membrane protein . Melanosome . Vesicular, endosomal like structures.