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Mouse IL-20/Interleukin-20 ELISA Kit PicoKine®, Carrier-free

Mouse IL-20 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il20 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

IL-20, also known as Interleukin 20 or Zcyto10, is a protein that in humans is encoded by the IL20 gene. This gene is mapped to 1q32.1. IL-20 is a protein belonging to the IL-10 family of cytokines. This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>93</td><td>89</td><td>90</td><td>83</td><td>88</td><td>3.63</td><td>4.1%</td></tr><tr><td>Sample 2</td><td>232</td><td>246</td><td>237</td><td>225</td><td>235</td><td>7.64</td><td>3.2%</td></tr><tr><td>Sample 3</td><td>997</td><td>874</td><td>895</td><td>860</td><td>906</td><td>53.71</td><td>5.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-20;IL-20;Cytokine Zcyto10;Il20;Zcyto10;

Gene Name

Interleukin-20

Gene ID

58181

UniProt

Q9JKV9

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L25-E176

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20098 MW

Specificity

Natural and recombinant mouse IL-20

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Protein Name

Interleukin-20

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