Mouse CD70/TNFSF7/cd27L ELISA Kit PicoKine®, Carrier-free

Mouse CD70/TNFSF7/cd27L ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd70 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD70 (Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24% amino acid sequence identity in the extracellular region.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>79</td><td>82</td><td>85</td><td>75</td><td>80</td><td>3.69</td><td>4.6%</td></tr><tr><td>Sample 2</td><td>248</td><td>239</td><td>232</td><td>248</td><td>241</td><td>6.72</td><td>2.7%</td></tr><tr><td>Sample 3</td><td>791</td><td>821</td><td>875</td><td>932</td><td>854</td><td>53.8</td><td>6.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

CD70 antigen;CD27 ligand;CD27-L;Tumor necrosis factor ligand superfamily member 7;CD70;Cd70;Cd27l, Cd27lg, Tnfsf7;

Gene Name

CD70 antigen

Gene ID

21948

UniProt

O55237

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S45-P195

Tissue Specificity

Very low level of expression. Detected in splenocytes and thymocytes.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)