Rat HGF/Hepatocyte growth factor ELISA Kit PicoKine®, Carrier-free

Rat HGF/Hepatocyte growth factor ELISA Kit PicoKine® (96 Tests). Quantitate Rat Hgf in cell culture supernatants, serum and plasma (EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000.1 The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus.2 HGF may serve as a paracrine mediator to control placental development and growth.3 This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin.4 The gene encoding the human HGF is assigned to human chromosome 7.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>223</td><td>205</td><td>208</td><td>224</td><td>215</td><td>8.57</td><td>3.9%</td></tr><tr><td>Sample 2</td><td>1031</td><td>1143</td><td>985</td><td>1076</td><td>1058</td><td>58.31</td><td>5.5%</td></tr><tr><td>Sample 3</td><td>4762</td><td>4701</td><td>5150</td><td>5432</td><td>5011</td><td>297.76</td><td>5.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Hepatocyte growth factor;Hepatopoietin-A;Scatter factor;SF;Hepatocyte growth factor alpha chain;Hepatocyte growth factor beta chain;Hgf;

Gene Name

Hepatocyte growth factor

Gene ID

24446

UniProt

P17945

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q33-R495 (alpha) & V496-L728 (beta)

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (EDTA, citrate).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (By similarity). .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)