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Mouse Galectin-7/LGALS7 ELISA Kit PicoKine®, Carrier-free

Mouse Galectin-7/LGALS7 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Lgals7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Galectin-7, also known as GAL7, is a protein that in humans is encoded by the LGALS7 gene. This gene is mapped to 19q13.2. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It has been found that expression of Galectin-7 was markedly upregulated in corneal epithelium after injury and that exogenous galectin-7 stimulated reepithelialization of corneal wounds.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>161</td><td>138</td><td>155</td><td>158</td><td>153</td><td>8.91</td><td>5.8%</td></tr><tr><td>Sample 2</td><td>861</td><td>902</td><td>914</td><td>909</td><td>896</td><td>20.93</td><td>2.3%</td></tr><tr><td>Sample 3</td><td>2074</td><td>2175</td><td>2110</td><td>2264</td><td>2155</td><td>72.22</td><td>3.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Galectin-7;Gal-7;Lgals7;

Gene Name

Galectin-7

Gene ID

16858

UniProt

O54974

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S2-F136

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

93.8 pg/ml - 6,000 pg/ml

Sensitivity

<10 pg/ml

Function

Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release (By similarity). .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

15173 MW

Specificity

Natural and recombinant mouse Galectin-7

Anticoagulant

heparin or EDTA

Subcellular Location

Cytoplasm . Nucleus . Secreted . May be secreted by a non-classical secretory pathway. .

Protein Name

Galectin-7

Curated Selection

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