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Human Galectin-2 ELISA Kit PicoKine®, Carrier-free

Human Galectin-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Galectin-2 is a protein that in humans is encoded by the LGALS2 gene. It is mapped to 22q13.1. The protein encoded by this gene is a soluble beta-galactoside binding lectin. The encoded protein is found as a homodimer and can bind to lymphotoxin-alpha. It has been found that a single-nucleotide polymorphism in Galectin-2 is significantly associated with susceptibility to myocardial infarction. This genetic substitution affects the transcriptional level of Galectin-2 in vitro, potentially leading to altered secretion of LTA, to which Galectin-2 binds, which would then affect the degree of inflammation.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>288</td><td>323</td><td>331</td><td>326</td><td>317</td><td>16.98</td><td>5.3%</td></tr><tr><td>Sample 2</td><td>1975</td><td>1974</td><td>1898</td><td>1989</td><td>1959</td><td>35.71</td><td>1.8%</td></tr><tr><td>Sample 3</td><td>5128</td><td>5263</td><td>5282</td><td>4554</td><td>5056</td><td>296.27</td><td>5.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Galectin-2;Gal-2;Beta-galactoside-binding lectin L-14-II;HL14;Lactose-binding lectin 2;S-Lac lectin 2;LGALS2;

Gene Name

Galectin-2

Gene ID

3957

UniProt

P05162

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-E132

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

This protein binds beta-galactoside. Its physiological function is not yet known.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

14644 MW

Specificity

Natural and recombinant human Galectin-2

Anticoagulant

heparin or EDTA

Sequence Similarities

Contains 1 galectin domain.

Protein Name

Galectin-2

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