Mouse Prostasin ELISA Kit PicoKine®, Carrier-free

Mouse Prostasin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Prss8 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60% to 80% increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1504</td><td>1621</td><td>1563</td><td>1509</td><td>1549</td><td>47.44</td><td>3%</td></tr><tr><td>Sample 2</td><td>6502</td><td>6771</td><td>6290</td><td>6265</td><td>6457</td><td>203.33</td><td>3.1%</td></tr><tr><td>Sample 3</td><td>26206</td><td>23293</td><td>23327</td><td>24983</td><td>24452</td><td>1221.41</td><td>4.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Prostasin;3.4.21.-;Channel-activating protease 1;CAP1;Serine protease 8;Prostasin light chain;Prostasin heavy chain;Prss8;Cap1;

Gene Name

Prostasin

Gene ID

76560

UniProt

Q9ESD1

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A30-Q289

Applications

ELISA

Standard

50 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

0.78 ng/ml - 50 ng/ml

Sensitivity

<50 pg/ml

Function

Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates (By similarity). Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G). .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)