Human Ficolin-2 ELISA Kit PicoKine®, Carrier-free

Human Ficolin-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human FCN2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Ficolin-2, which was initially identified as L-ficolin, is a protein that in humans is encoded by the FCN2 gene. The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. It is mapped to 9q34.3. This gene is predominantly expressed in the liver, and has been shown to have carbohydrate binding and opsonic activities. Serum concentration of ficolin-2 varies considerably in healthy individuals. FCN2 promoter polymorphisms were associated with marked changes in theficolin-2 serum concentration.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>977</td><td>1058</td><td>1110</td><td>1008</td><td>1038</td><td>50.5</td><td>4.8%</td></tr><tr><td>Sample 2</td><td>6812</td><td>6337</td><td>6515</td><td>7162</td><td>6706</td><td>312.97</td><td>4.6%</td></tr><tr><td>Sample 3</td><td>22201</td><td>22603</td><td>22446</td><td>21704</td><td>22238</td><td>340.22</td><td>1.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Ficolin-2;37 kDa elastin-binding protein;Collagen/fibrinogen domain-containing protein 2;EBP-37;Ficolin-B;Ficolin-beta;Hucolin;L-ficolin;Serum lectin p35;FCN2;FCNL;

Gene Name

Ficolin-2

Gene ID

2220

UniProt

Q15485

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L26-A313

Tissue Specificity

Expressed by the liver and secreted in plasma.

Applications

ELISA

Standard

50 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin).

Detection Range

0.78 ng/ml - 50 ng/ml

Sensitivity

<20 pg/ml

Function

May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)