Human Lumican ELISA Kit PicoKine®, Carrier-free

Human Lumican ELISA Kit PicoKine® (96 Tests). Quantitate Human LUM in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Lumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene (SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. Lumican is mapped to 12q21.33, it interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate-containing glycosaminoglycan side chains LDC.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>349</td><td>325</td><td>293</td><td>350</td><td>329</td><td>23.19</td><td>7%</td></tr><tr><td>Sample 2</td><td>1777</td><td>1805</td><td>2029</td><td>1826</td><td>1859</td><td>99.53</td><td>5.3%</td></tr><tr><td>Sample 3</td><td>5103</td><td>4485</td><td>4831</td><td>4805</td><td>4806</td><td>219.01</td><td>4.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Lumican;Keratan sulfate proteoglycan lumican;KSPG lumican;LUM;LDC, SLRR2D;

Gene Name

Lumican

Gene ID

4060

UniProt

P51884

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q19-N338

Tissue Specificity

Cornea and other tissues.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)