Human SPINK1/TATI ELISA Kit PicoKine®, Carrier-free

Human SPINK1/TATI ELISA Kit PicoKine® (96 Tests). Quantitate Human SPINK1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Serine protease inhibitor Kazal-type 1, also called TATI or PSTI, is a protein that in humans is encoded by the SPINK1 gene. It is mapped to 5q32. SPINK1 gene contains 4 exons spanning approximately 7.5 kb. It was initially detected in urine of patients with ovarian cancer. This peptide is also produced by the mucosa of the gastrointestinal tract where it may protect the mucosal cells from proteolytic breakdown. The SPINK1 gene encodes pancreatic secretory trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. Its physiologic role was thought to be the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>245</td><td>238</td><td>239</td><td>225</td><td>236</td><td>7.29</td><td>3%</td></tr><tr><td>Sample 2</td><td>1862</td><td>1592</td><td>1831</td><td>1832</td><td>1779</td><td>108.82</td><td>6.1%</td></tr><tr><td>Sample 3</td><td>5817</td><td>5596</td><td>5823</td><td>5918</td><td>5788</td><td>118.14</td><td>2%</td></tr></table>*number of samples for each test n=16.

Synonyms

Pancreatic secretory trypsin inhibitor;Serine protease inhibitor Kazal-type 1;Tumor-associated trypsin inhibitor;TATI;SPINK1;PSTI;

Gene Name

Pancreatic secretory trypsin inhibitor

Gene ID

6690

UniProt

P00995

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D24-C79

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

This is a trypsin inhibitor, its physiological function is to prevent the trypsin-catalyzed premature activation of zymogens within the pancreas.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)