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Mouse Relaxin 1 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1231-carrier-free

Size: 96 Well With Removable Strips

Mouse Relaxin 1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Rln1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to mouse chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>104</td><td>106</td><td>113</td><td>116</td><td>109</td><td>4.91</td><td>4.5%</td></tr><tr><td>Sample 2</td><td>612</td><td>581</td><td>605</td><td>608</td><td>601</td><td>12.09</td><td>2%</td></tr><tr><td>Sample 3</td><td>2407</td><td>2396</td><td>2116</td><td>2524</td><td>2360</td><td>149.94</td><td>6.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Prorelaxin 1;Relaxin B chain;Relaxin A chain;Rln1;Rln, Rlx;

Gene Name

Prorelaxin 1

Gene ID

19773

UniProt

P47932

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R23-R54 (B) & E161-C185 (A)

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

78 pg/ml - 5,000 pg/ml

Sensitivity

<10 pg/ml

Function

Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20571 MW

Specificity

Natural and recombinant mouse Relaxin 1

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Protein Name

Prorelaxin 1

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