Human Cystatin B/CSTB ELISA Kit PicoKine®, Carrier-free

Human Cystatin B ELISA Kit PicoKine® (96 Tests). Quantitate Human CSTB in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cystatin B (CSTB), also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is thought to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>108</td><td>102</td><td>101</td><td>101</td><td>103</td><td>2.91</td><td>2.8%</td></tr><tr><td>Sample 2</td><td>522</td><td>468</td><td>537</td><td>457</td><td>496</td><td>34.13</td><td>6.8%</td></tr><tr><td>Sample 3</td><td>1824</td><td>1767</td><td>1982</td><td>1870</td><td>1860</td><td>78.94</td><td>4.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

Cystatin-B;CPI-B;Liver thiol proteinase inhibitor;Stefin-B;CSTB;CST6, STFB;

Gene Name

Cystatin-B

Gene ID

1476

UniProt

P04080

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M2-F98

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)