Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®, Carrier-free

Human CFD/Adipsin ELISA Kit PicoKine® (96 Tests). Quantitate Human CFD in cell culture supernatants, cell lysates, serum, plasma(heparin , EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Complement factor D (CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What's more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>787</td><td>751</td><td>817</td><td>732</td><td>771</td><td>32.75</td><td>4.2%</td></tr><tr><td>Sample 2</td><td>2378</td><td>2440</td><td>2261</td><td>2414</td><td>2373</td><td>68.44</td><td>2.8%</td></tr><tr><td>Sample 3</td><td>9095</td><td>10188</td><td>9364</td><td>8739</td><td>9346</td><td>534.02</td><td>5.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Complement factor D;3.4.21.46;Adipsin;C3 convertase activator;Properdin factor D;CFD;DF, PFD;

Gene Name

Complement factor D

Gene ID

1675

UniProt

P00746

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I26-A253

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum, plasma(heparin , EDTA) and urine.

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Function

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)