Mouse Periostin/OSF2 ELISA Kit PicoKine®, Carrier-free

Mouse Periostin/OSF2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Postn in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/ beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>109</td><td>109</td><td>111</td><td>107</td><td>109</td><td>1.41</td><td>1.2%</td></tr><tr><td>Sample 2</td><td>795</td><td>840</td><td>784</td><td>883</td><td>825</td><td>39.27</td><td>4.7%</td></tr><tr><td>Sample 3</td><td>2510</td><td>2434</td><td>2494</td><td>2308</td><td>2436</td><td>79.41</td><td>3.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

Periostin;PN;Osteoblast-specific factor 2;OSF-2;Postn;Osf2;

Gene Name

Periostin

Gene ID

50706

UniProt

Q62009

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: N24-Q836

Tissue Specificity

Preferentially expressed in periosteum and periodontal ligament. Also expressed in the developing and adult heart. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

93.7 pg/ml - 6,000 pg/ml

Sensitivity

<10 pg/ml

Function

Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)